Erythrocyte Pyrimidine 5'-Nucleotidase Test for Occupational Lead Exposure

Erythrocyte arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N) as indices of lead exposure.

The activities of three erythrocyte (rbc) enzymes, arginase, pyrimidine 5'-nucleotidase (P5N), and deoxypyrimidine 5'-nucleotidase (dP5N), were compared in 16 lead workers and 14 age matched controls as correlates of blood lead (PbB) and unextracted zinc protoporphyrin (EP) concentrations. Subjects with PbB of 0.9-2.5 microM (19-52 micrograms/dl) had 6.5 +/- 0.6 IU of P5N activity with uridine ...

Effects of low-level lead exposure on pyrimidine 5'-nucleotidase and other erythrocyte enzymes. Possible role of pyrimidine 5'-nucleotidase in the pathogenesis of lead-induced anemia.

Similarities between lead-induced anemia and a new hereditary erythorenzymopathy involving pyrimidine-specific 5'-nucleotidase prompted studies of the effects of lead on this and other erythrocyte enzymes. In vitro incubations of normal mature erythrocytes demonstrated that significant inhibition of pyrimidine 5'-nucleotidase occurred in the presence of lead at concentrations that had minimal e...

A simplified method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N) activity by HPLC and its value in monitoring lead exposure.

The method for determining erythrocyte pyrimidine 5'-nucleotidase (P5N EC 3.1.3.5) activity has been simplified using an automated high performance liquid chromatograph (HPLC). The activity determined by the simplified method agreed closely with that obtained by conventional methods. In 161 lead workers P5N activity declined linearly with increasing blood lead concentrations (Pb-B) between 20 a...

Response of erythrocyte pyrimidine 5'-nucleotidase (P5N) activity in workers exposed to lead.

Effects of lead exposure on the activity of erythrocyte pyrimidine 5'-nucleotidase and delta-aminolevulinic acid dehydratase in mice.

T0M0KUNI, K. Effects of Lead Exposure on the Activity of Erythrocyte Pyrimidine 5'-Nucleotidase and s-Aminolevulinic Acid Dehydratase in Mice. Tohoku J. exp. Med., 1983, 139 (1), 27-32 The effects of lead exposure on the activities of erythrocyte pyrimidine 5'-nucleotidase (Py5N) and s-aminolevulinic acid dehydratase (ALA-D) were investigated in the mice given ad libitum the drinking water cont...